Recombinant:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
Plasmid Preparation:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
Polymerase Chain Reaction:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
DNA Extraction:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
SYBR Green Assay:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
Flow Cytometry:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
Binding Assay:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
Sample Prep:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
Reverse Transcription:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
Transfection:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
Amplification:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
Sequencing:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
RNA Sequencing:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
CMC:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
Software:Article Title: AI-generated MLH1 small binder improves prime editing efficiency.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 10X TBS Solution, pH 7.4 Welgene Cat#ML023-03 TWEEN® 20 Sigma-Aldrich Cat#P1379-100ML Proteinase K (Recombinant), Molecular/PCR Grade, AG scientific Cat#P-1264-100MG TRIS (Ultrapure) LPS solution Cat#T1503-1KG Nonidet® P 40 Substitute (NP-40), Reagent Grade Avantor Cat#E109-100ML Critical commercial assays ExprepTM Plasmid SV, 200prep GeneAll Cat#101-102 ExpinTM Gel SV, 200prep GeneAll Cat#102-102 ExpinTM PCR SV, 200prep GeneAll Cat#103-102 NucleoBond Xtra Midi EF, Midi kit MACHEREY-NAGEL Cat#740420.5 AccuPrep® Genomic DNA Extraction Kit Bioneer CatK-3032 PrimeScriptTM RT Master Mix (Perfect Real Time) Takara Cat#RR036A iTaq Universal SYBR Green Supermix Bio-Rad Cat#1725124 Gibson Assembly® Master Mix NEB Cat#E2611 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry Thermo Fisher Scientific Cat#V13241 FOXP3 Fix/Perm Buffer Set BioLegend Cat#421403 Annexin V binding buffer BioLegend Cat#422201 SMARTer Ultra-Low Input RNA Sample Prep Kit Clontech Cat#634940 SMARTScribeTM Reverse Transcriptase Clontech Cat#639536 NeonTM Transfection System Thermo Fisher Scientific Cat#MPK1096 MiniSeqTM Mid Output Kit (300 cycles) Illumina Cat#FC-420-1004 Deposited data Amplicon sequencing data This paper NCBI SRA: BioProject PRJNA1229090 Whole-genome sequencing data This paper NCBI SRA: BioProject PRJNA1229090 RNA-seq data This paper NCBI SRA: BioProject PRJNA1229090 Experimental models: Cell lines Human (female): HEK293T ATCC Cat#CRL-3216 Human (female): HeLa ATCC Cat#CCL-2 Human (female): HEK293 ATCC Cat#CRL-1573 Primary human fibroblast Seoul National University Hospital IRB-H-0905-041-281 human iPSC (female) Catholic University of Korea CMC-hiPSC-022 Experimental models: Organisms/strains Female Institute of Cancer Research (ICR) mice Orient Bio Cat#2160272 Recombinant DNA pCMV-PE2 Addgene Addgene plasmid #132775 pCMV-PEmax Addgene Addgene plasmid #174820 pCMV-PE6b Addgene Addgene plasmid #207852 pCMV-PE6c Addgene Addgene plasmid #207853 pCMV-PE6d Addgene Addgene plasmid #207854 pCMV-PuroR-2A-MLH1-SB This paper Addgene plasmid #228871 pCMV-PE7-SB This paper Addgene plasmid #228869 pCMV-PE7-SB-NLS This paper Addgene plasmid #228870 pCMV-PE7-SB2 This paper Addgene plasmid #228868 Software and algorithms Cas-Analyzer Park et al. 52 http://www.rgenome.net/cas-analyzer/ (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–16.e1–e6, October 16, 2025 Please cite this article in press as: Park et al., AI-generated MLH1 small binder improves prime editing efficiency, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.07.010 Article
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